Using NextGenPCR® SARS-CoV-2 detection method

PCR detection of SARS-CoV-2 is based on Multiplex RT-PCR. MBS has developed an ultra-fast SARS-CoV-2 PCR detection using NextGenPCR®. This detection works without RNA extraction, thus performs a PCR directly on the sample. After a heat inactivation step, the sample is added to the RT-PCR mix in a NextGenPCR® plate. Subsequently, this plate then cycles in a NextGenPCR® thermal cycler.

The assay amplifies human RNAseP as an internal control and 2 fragments of the Coronavirus, being one of the Orf1ab gene and one of the N-gene. The assay labels the RNAseP amplicons with cy5. We label both of the Coronavirus amplicons with FAM. Therefore, we label both Coronavirus amplicons with the same label. Thus, we amplify the detection power for a lower limit of detection. The assay detects RNAseP amplicons separately. This way you can confirm successful sampling and PCR amplification.

NextGenPCR SARS-CoV-2 detection kit

The NextGenPCR® SARS-CoV-2 detection assay contains a primer-probe mix for the multiplex RT-PCR, which is a ready-to-use enzyme mix with buffer and dNTP. The assay also contains RNAse-free water and is especially resistant to inhibitors commonly found in plant and animal tissue. Because of it’s exceptional speed, the enzyme functions optimally with the ultra fast NextGenPCR® thermal cycler. This cycler effectively eliminates ramping times.

Because of the above, customers successfully employ NextGenPCR® Corona virus detection where the circumstances require ultra high speed and/or high-throughput.

Peer-reviewed publication detailing assay performance
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list of features

• Direct extraction-free PCR
• 27 min RT-PCR
• Highly sensitive RT-PCR detection: 1 copy per microliter
• Excellent concordance to referenced qPCR methods
• Multi-target design detects all variants
• Suitable for containerized laboratory use