27-minute detection of SARS-CoV-2
Since the beginning of the pandemic, Molecular Biology Systems started with the development of an application for the detection of SARS-CoV-2. The aim of this research is to develop a cost efficient, fast, high-throughput and flexible method for the detection of SARS-CoV-2.
The SARS-CoV-2 test is based on Multiplex RT-PCR. Three amplifications are carried out simultaneously in the presence of SARS-CoV-2 RNA and human genomic DNA. The detection of SARS-CoV-2 RNA is covered by two amplicons, one specific for Orf1ab and the other specific for the N-gene. The presence of human genomic DNA is indicated by amplification of the human RNase P, confirming that sampling and extraction were successful.
The target sequences of the amplified DNA are detected by target sequence specific probes. The probes are labelled with fluorescent reporter and quencher dyes. The human RNase P probe is labelled with Cy5, the SARS-CoV-2 specific probes are labelled with FAM on their 5-prime ends.
These primers and probes are combined with a ready to use master mix for rapid detection of SARS-CoV-2. This NextGenPCR® master mix is certified by MBS to be fast and robust for PCR inhibitors commonly found in plant and animal tissues. This makes is possible to use a direct PCR method combined with an ultra-fast PCR system.