NextGenPCR® | From hours to minutes | High throughput | Any assay

NextGenPCR® Sanger Sequencing

The NextGenPCR® technology can be used with a plethora of different downstream genetic analyses protocols to identify nucleotide variations in one or more genomic target(s) of interest. The classic Sanger chain termination sequencing approach, in combination with capillary electrophoresis, is no exception. With the ultrafast chemistry and instrumentation that NextGenPCR® provides, it only takes minutes to amplify your target which can then be sequenced on a genome analyzer of choice resulting in high fidelity and robust sequencing data.

  • BRCA1 amplicon generation in less than 10 minutes
  • NextGenPCR® high fidelity chemistry allows for high quality sequencing data
  • PCR can be performed directly on crude samples* without nucleic acid extraction needed

* PCR inhibitory substances may influence amplicon generation during cycling. Caution is advised if your sample type includes any known PCR inhibitors.

Please get in touch to discuss how Ultra-fast PCR can make the difference in your Sanger Sequencing application!

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