- 1. Does NEXTGENPCR hold samples at 4 degrees after PCR?More
Overnight PCR is usually not necessary, as the instrument performs PCR reaction in well under 10 minutes. Overnight use can be enabled, by use of a robot and robotic access to a refrigerator. The instrument can then continuously work through the night.
- 2. How much does it cost?
- 3. What mastermix/enzyme do I have to use?
- 4. Do I have to use special plates?More
Yes, only NEXTGENPCR microplates should be used in the NEXTGENPCR machine. All microplates have wells with a wall thickness of less than 50 microns, in comparison to normal pcr tube with a thickness of approximately 300 microns. The temperatures inside each reaction change almost instantly due to the heat transfer across these thin-walled microplates.
- 5. Can I use strip tubes?More
No, only MBS microplate can be used.
- 6. What reaction volume will fit in a well?More
96-well plates may be used with volumes from 5 – 20 or 2-5 microliters.
48-well plates may be used with volume from 20-50 microliters.
24-well plates may be used with volumes from 20-50 microliters.
384-well plates may be used with volumes from 2-5 microliters.