cyQlone™ – Quantitative PCR at lightning speed!

Enhance your NextGenPCR™ workflow with the launch of cyQlone™, our blazing fast qPCR, a quantitative version of the NextGenPCR™ Thermal Cycler that allows analysis of PCR fragments in real-time.

Our new cyQlone™ instrument is the next evolution of NextGenPCR™ thermal cycling technology. The instrument is equipped with a cleverly engineered optical module that allows for powerful 6-channel multiplexing. The innovative optics system performs extremely rapid fluorescence measurements, averaging a detection time of 20 milliseconds per channel per cycle. A full 96-well microplate can be amplified and analyzed in as little as 9 minutes, making cyQlone™ the fastest SBS-format qPCR instrument on the planet.

But the capabilities of cyQlone™ go beyond its industry-leading speed. By maintaining three constant temperatures instead of temperature ramping, temperature under- and overshoot is prevented, contributing to excellent precision. In addition, precision is enhanced by the compression of the microplate during cycling, which produces highly uniform temperature distribution throughout the wells.

Another key benefit of cyQlone™ is its focus on supporting more sustainable laboratory workflows. Its low energy consumption (200 W) helps laboratories to reduce overall power usage. The system further minimizes environmental impact through proprietary SBS-format microplates that contain up to 75% less plastic than standard PCR microplates. Moreover, cyQlone™ supports reaction volumes from 0.2-20 µL, minimizing reagent consumption per assay.

cyQlone™ is a semi-open instrument expected to be compatible with a broad range of existing probe-based and intercalating dye RT-qPCR chemistries, facilitating straightforward integration into established laboratory workflows. Its space-saving design, measuring just under 20 cm in width, ensures no valuable bench space is compromised.

Benefits of cyQlone™

• Versatile qPCR setup: 6 channel detection capability
• Generate accurate results: ultrafast and precise temperature control
• Resource efficient: 0.2-20 µL reaction range
• Environment friendly: saves energy and produces less plastics waste
• Easy integration: space-saving design and compatibility with existing assays

Technical Specifications

Optical range for compatible dyes

Applications

Applications for quantitative PCR cover a wide range of life science fields. cyQlone™ Can help in accelerating turnaround times for countless quantitative real-time PCR applications.

Pathogen detection

In clinical research rapid outcomes of molecular tests are crucial for adequate patient care. Quantitative PCR is used to for example detect pathogenic species in clinical samples or investigate gene expression of human tissues to detect disease states. Obtaining a fast diagnosis leads to better clinical decision making for available treatments, less waiting times and improved patient outcomes.

Microbial surveillance

With the ever increasing threat of emerging pandemics, genome surveillance of microbial strains is becoming a focal point of microbiologists around the globe. Rapid microbial characterization methods are vital for mediating outbreak threats, generating insights into circulating microbial strains and guide public health decisions.

A preview of the performance of cyQlone™ in microbial surveillance and our first assay kit for qPCR (IAV detection) can be found here.

Plant & animal genotyping

Genetics studies for plant and animal breeding purposes have historically been employed to increase yields and improve resistance to diseases and changing environmental conditions. Breeding applications are characterized by large sample numbers that require high-throughput setups, and are dependent on generating data quickly to streamline continued breeding potential.

Successful amplification of low-volume reactions

Let’s get started as soon as possible:

To inquire about our blazing fast qPCR system cyQlone™, please contact us via the contact form below.