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NextGenPCR® Pre mixed Monkeypox sequencing primer pools (500 samples)
To aid laboratories with their Monkeypox virus ( surveillance effort, this product contains pre mixed Monkeypox sequencing primer pools for a total of 500 samples. Amplification of the 190 kb MPXV genome was achieved by using a PCR based amplicon tiling of ~2.5kb segments (88 amplicons total). With NextGenPCR® ultra fast PCR chemistry, the complete PCR cycling program of the protocol can be completed in less than 45 minutes by using the NextGenPCR® thermocycler.
- OVERVIEW
- HANDLING INSTRUCTIONS
- SPECIFICATIONS
- CONTACT US
OVERVIEW
Contents:
- 2x 275 µl primer pool 1
- 2x 275 µl primer pool 2
- 1x 2.0 ml Nuclease free water
HANDLING INSTRUCTIONS
- It is recommended to use NextGenPCR ® Arctic Fox HF Chemistry 2x ( 50050; ready to use mastermix)
- Do not use past the expiration date. – Mark tubes already in use.
- When working with RNA and DNA, appropriate precautions should be taken to avoid the introduction of nucleases.
- Use aerosol barrier pipet tips for pipetting.
- Freezing small aliquots is the optimal storage condition when performing fewer reactions.
- Store away from any source of contaminating DNA, especially amplified DNA products.
Safety Information
Use appropriate laboratory safety practices and personal protective equipment when working with this reagent.
SPECIFICATIONS
Catalog Number | 50026 |
Storage Conditions | Store the Monkeypox sequencing primer pools (250 samples) at 20°C |
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