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NextGenPCR ® Monkeypox sequencing library prep kit (250 samples)

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NextGenPCR ® Monkeypox sequencing library prep kit 250 samples)

To aid laboratories with their Monkeypox virus ( surveillance effort, this library prep kit contains pre mixed Monkeypox sequencing primer pools (for a total of 250 samples) and the NextGenPCR® Arctic Fox HF Chemistry 2x (ready to use mastermix). Amplification of the 190 kb MPXV genome was achieved by using a PCR based amplicon tiling of ~2.5kb segments (88 amplicons total). With NextGenPCR® ultra fast PCR chemistry, the complete PCR cycling program of the protocol can be completed in less than 45 minutes by using the NextGenPCR® thermocycler.

OVERVIEW

OVERVIEW

Contents:

  • 1x 275 µ l primer pool 1
  • 1x 275 µ l primer pool 2
  • 4x 1.25 ml NextGenPCR ® Arctic Fox HF Chemistry 2x
  • 1x 2.0 ml Nuclease free water
HANDLING INSTRUCTIONS

HANDLING INSTRUCTIONS

  • Do not use past the expiration date.
  • Mark tubes already in use.
  • When working with RNA and DNA, appropriate precautions should be taken to avoid the introduction of nucleases.
  • Use aerosol barrier pipet tips for pipetting.
  • Freezing small aliquots is the optimal storage condition when performing fewer reactions.
  • Store away from any source of contaminating DNA, especially amplified DNA products.

Safety Information

Use appropriate laboratory safety practices and personal protective equipment when working with this reagent.

SPECIFICATIONS

SPECIFICATIONS

Catalog Number 50027
Storage Conditions Store the Monkeypox sequencing library prep kit (250 samples) at 20°C.

 

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