Since the introduction of PCR many people have tried to increase the speed of PCR. Ramp rates go up to 15 degrees per second, and chemistry is getting faster and faster. The NEXTGENPCR device will heat and cool the samples instantly, loosing no time getting the samples on the desired temperature. Ramp rates are an estimated 1000 degrees per second. For the first time, the machine is not the limitation, but the chemistry is.
Wells are formed of very thin polypropylene foil and attached to SBS compliant rigid plastic frames. After filling these wells with PCR mix, all wells are sealed using a proprietary anvil and a standard heat sealer. All plates are shaped to readily integrate into laboratory automation.
When microplates arrives at a temperature zone, the two temperature blocks are brought together and slightly compress the wells. This process mixes the liquid in each well. Together with the large surface to volume ratio this enables a practically instantaneous change of one temperature to the next.
Sharp polypropylene pipet tips enable piercing of even polyester backed foil. A special holder will prevent tips from piercing the bottom of the wells.
The NEXTGENPCR performs PCR within 2 minutes. Due to instant temperature changing between denaturation, annealing and extension, the only limitation of the speed is the polymeraze used.
Reducing the PCR time will result in higher sample throughput. Using optimised applications, more than 10 full plate experiments per hour become possible. In the ideal situation this would mean 80 384 well plates per days, resulting in more than 30000 datapoints per day.
Reduced number of instruments
Due to the higher throughput, laboratories can reduce their number of machines needed. This results in lower costs for purchase and maintenance.
Low energy consumption
One machine uses 150 Watts, rather than 500 Watts. The NEXTGENPCR machine is also up-to 30 times faster. This results in up-to 600 times less energy use per experiment.